Transformation of the dermatophyte Trichophyton mentagrophytes to hygromycin B resistance
نویسندگان
چکیده
منابع مشابه
Rapid method for differentiation of Trichophyton rubrum, Trichophyton mentagrophytes, and related dermatophyte species.
Bromocresol purple-milk solids-glucose medium, proposed by Fischer and Kane in 1971 (Mycopathol. Mycol. Appl. 43:169-180, 1971) as an aid in the rapid determination of Trichophyton rubrum and Trichophyton mentagrophytes, was evaluated across a wide range of isolates to determine its accuracy and efficacy in the clinical laboratory. Results showed that it facilitated accurate determination of ty...
متن کاملSurvival and resistance of Trichophyton mentagrophytes arthrospores.
The effects of several physical and chemical agents on the survival of Trichophyton mentagrophytes arthrospores were investigated. Although arthrospores of this dermatophyte were highly resistant to chilling and freezing, they were extremely susceptible to moderate heat (above 50 degrees C) and desiccation. This high susceptibility could be significantly reduced when they were dried in the pres...
متن کاملDermatophyte isolation media: quantitative appraisal using skin scales infected with Trichophyton mentagrophytes and Trichophyton rubrum.
Various dermatophyte isolation media were quantitatively appraised by colony counts for their ability to support growth when suspensions of trypsin-treated infected-skin scales were used as inocula. Evaluation included guinea pig skin scales infected with a granular Trichophyton mentagrophyton mentagrophytes, and one trial was conducted using human skin scales infected with Trichophyton rubrum....
متن کاملGreen fluorescent protein (GFP) as a vital marker for pathogenic development of the dermatophyte Trichophyton mentagrophytes.
Skin infections by dermatophytes of the genus Trichophyton are widespread, but methods to investigate the molecular basis of pathogenicity are only starting to be developed. The initial stages of growth on the host can only be studied by electron microscopy, which requires fixing the tissue. This paper shows that restriction-enzyme-mediated integration (REMI) provides stable expression of the g...
متن کاملTLR2−/− Mice Display Increased Clearance of Dermatophyte Trichophyton mentagrophytes in the Setting of Hyperglycemia
Dermatophytosis is one of the most common human infections affecting both immunocompetent individuals and immunocompromised patients, in whom the disease is more aggressive and can reach deep tissues. Over the last decades, cases of deep dermatophytosis have increased and the dermatophyte-host interplay remains poorly investigated. Pattern recognition molecules, such as Toll-like receptors (TLR...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1989
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.57.9.2923-2925.1989